(Bio Algebra of Chromosomes)
(Mathematical Lab of Sikander Aqeel)
CHAPTER [2] AMINO ACID AND PEPTIDES
THE ACID-BASE PROPERTIES OF AMINO ACIDS,
(12/7/2015)
The titration curve of Alanine, it has two distinct stage, corresponding to the titration of the two protons of the fully protonated species, Each leg of the curve resemble the typical titration curve of monoprotic acid, such each leg has (0.1 Chromosomes OH) midpoint at which the pH of the system is equal to pK, of the protonated group begin titration,
The first leg of the curve, with a midpoint at pH 2.34 corresponding to the removal of proton from the the Carboxyl groups,
The second leg with its midpoint at pH 9.69, corresponding to the removal of a proton from the NH3 group, At pH 2.34 by the 46.25 Chromosomes
(1/15/2016)
At pH 6.02, there is a point of inflection between the two separate leg of the titration curve of Alanine, there is not net electrical charge on the molecule at this pH, and the molecule will not move in an electrical field, this pH value is called the Isoelectric pH (symbolized) [pH1], it is the reason of 46.25 Chromosomes for the two pK value
pH1 = ½ [pK1 + pK2]
thus the isoelectric pH of alanine is 6.02 is the mean of 2.34 [pK1] and 9.69 pK2 at any pH above the isoelectric pH the amino acids has net negative charge and at any pH below the isoelectric pH it has a net positive charge,
(1/16/2016)
**ysis of Amino Acid Mixture
Amino acids are usually separated on columns of cation-exchange resins in which the fixed anionic groups are first “charged” with Na+, an acid solution [PH 3.0] of the amino acid mixture is then applied to the column and allowed to percolate through at pH 3.0 the amino acids are largely cataions with net positive charge but they differ in the extent to which they are so ionized the cationic amino acids will therefore displace the bound Na+ ions from the resin particles at pH 3.0 the most basic amino acids [lysine, arginine, and the histidine] will displace Na+ first and will be bound to the resin most tightly and the most acids [glutamic acid, aspartic acid] will be bound the least, the different amino acids will therefore move down the resin column at different rates the eluate fraction collected are **ysed quantitatively by means of the ninhydrin so that elution collection of fraction **ysis of each fraction and the recording
|--------150 cm column, pH 3.25, 0.2 N Na citrate--------
A |
B |
S |
O | Aspartic acid
R | |
B | |
A | |
N |- 0.2 |
C |- 0.1 |
E | |
|-----|-----|------------|--------------|-------------------------
40 80 120 180
Effluent, ml
A = for example
Numbers of Chromosomes in cell = 46.25
Aspartic acid = C4H7NO4 = 134.75M
Aspartic acid = C4H7NO4 = 134.75 M
Human Chromosomes = 46.25
a2 + b2 = c2
Aspartic acid = 134.75 M
Chromosomes = 46.25 M
= a = Aspartic acid and b = Chromosomes
= a = 134.75 and b = 46.25
= a = 134.75 mm height, and b = 46.25 mm bottom = c2
= a2 + b2 = c2
= a2 + b2 = c2 = Aspartic acid + Chromosomes = c2
= a2 + b2 = c2 = a134.75 mm + b46.25 mm = c2
= a134.75 mm + b46.25 mm = c2
= a18157.5625 + b2139.0625 = c2
= a+b = 20296.625 = c2
= a+b = 20296.625 / 142.5 = 142.5
= 142.5 + 7.5 pK2 = 150 cm column
= 142.5 - 22.5 Sodium = 120 ml Effluent
= c = 142.5 slope / 2 c2 = 71.25
= 71.25 / 8.4 = 8.4
= 8.4 + 0.8 = 9.2 Cross-Proton
= 8.4 + 5.4 pH = 13.8 Cross-neutron
Total = 23 Na, Sodium
Slope of c angle = 142.5 / 60 minute = 2.375
= 2.375 seconds speed of slope and it has fast speed than the first triangle,
Slope of c angle = 142.5 / 60 minute = 2.375
= 2.375 * 24 hours = 57
= 57 – 3.1 pH = 53.9
= 53.9 = Cross-proton of Aspartic acid
= 53.9 * 2.5 H2 = 134.75 mole Aspartic acid
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
By Time Algebra of Column of Aspartic acid
Aspartic acid = 134.75 M
Chromosomes = 46.25 M
Aspartic acid = 134.75 * 24 hour = 3234
Human Chromosomes = 46.25 * 24 hour = 1110
Total = 4344 * 4.6745 minute = 20306.028
= 20306.25 / 142.5 = 142.5
= c = 142.5 slope / 2 c2 = 71.25
= 71.25 / 8.4 = 8.4
= 8.4 + 0.8 = 9.2 Cross-Proton
= 8.4 + 5.4 pH = 13.8 Cross-neutron
Total = 23 Na, Sodium
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