(Bio Algebra of Chromosomes)
(Mathematical Lab of Sikander Aqeel)
CHAPTER [2] AMINO ACID AND PEPTIDES
PEPTIDES,
(1/21/2016)
**ysis of Amino Acid Mixture
Amino acids are usually separated on columns of cation-exchange resins in which the fixed anionic groups are first “charged” with Na+, an acid solution [PH 3.0] of the amino acid mixture is then applied to the column and allowed to percolate through at pH 3.0 the amino acids are largely cataions with net positive charge but they differ in the extent to which they are so ionized the cationic amino acids will therefore displace the bound Na+ ions from the resin particles at pH 3.0 the most basic amino acids [lysine, arginine, and the histidine] will displace Na+ first and will be bound to the resin most tightly and the most acids [glutamic acid, Serine] will be bound the least, the different amino acids will therefore move down the resin column at different rates the eluate fraction collected are **ysed quantitatively by means of the ninhydrin so that elution collection of fraction **ysis of each fraction and the recording
|--------150 cm column, pH 3.25, 0.2, N, Na, citrate------------
A |
B |
S | Serine || Serine
O | Aspartate || Glutamic acid
R | | || |
B | | || | | proline
A | | || | |
N |- 0.2 | || | |
C |- 0.1 | || | |
E | | || | |
|-----|-----|------------|--------------||-----------|----------|---------
40 80 120 180 200 240
Effluent, ml
A = for example
Numbers of Chromosomes in cell = 46.25
Proline = C5H9NO2 = 117.25 M
Proline = C5H9NO2 = 117.25 M
Human Chromosomes = 46.25
a2 + b2 = c2
Proline = 117.25 M
Chromosomes = 46.25 M
= a = Proline and b = Chromosomes
= a = 117.25 and b = 46.25
= a = 117.25 mm height, and b = 46.25 mm bottom = c2
= a2 + b2 = c2
= a2 + b2 = c2 = Proline + Chromosomes = c2
= a2 + b2 = c2 = a117.25 mm + b46.25 mm = c2
= a117.25 mm + b46.25 mm = c2
= a13747.5625 + b2139.0625 = c2
= a + b = 15886.625 = c2
= a + b = 15886.625 / 126 = 126
= c = 126 slope / 2 c2 = 63
= 63 / 7.9 = 7.9
= 7.9 + 1.3 = 9.2 Cross-Proton
= 7.9 + 5.9 (Proline pH) = 13.8 Cross-neutron
Total = 23 Na, Sodium
= c = 126 slope + 24 % Proline = 150 cm column
Slope of c angle = 126 / 60 minute = 2.1
= 2.1 seconds speed of slope and it has fast speed than the first triangle,
Slope of c angle = 126 / 60 minute = 2.1
= 2.1 * 24 hours = 50.4
= 50.4 - 3.5 pH column = 46.9
= 46.9 = Cross-proton of Proline
= 46.9 * 2.5 H2 = 117.25 mole Proline
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
By Time Algebra of Column of Proline
Proline = 117.25 M
Chromosomes = 46.25 M
Proline = 117.25 * 24 hour = 2814
Human Chromosomes = 46.25 * 24 hour = 1110
Total = 3924 * 4.04587 minute = 15875.9
= 15876 / 126 = 126 slope
= c = 126 slope / 2 c2 = 63
= 63 / 7.9 = 7.9
= 7.9 + 1.3 = 9.2 Cross-Proton
= 7.9 + 5.9 (Proline pH) = 13.8 Cross-neutron
Total = 23 Na, Sodium
= c = 126 slope + 24 % Proline = 150 cm column
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