CHROMOSOMES WITH BIO-CHEMISTRY

(Bio Algebra of Chromosomes) 

(Mathematical Lab of Sikander Aqeel) 

CHAPTER [2] AMINO ACID AND PEPTIDES 

COLUMN, 

(1/30/2016) 

**ysis of Amino Acid Mixture 

Amino acids are usually separated on columns of cation-exchange resins in which the fixed anionic groups are first “charged” with Na+, an acid solution [PH 3.0] of the amino acid mixture is then applied to the column and allowed to percolate through at pH 3.0 the amino acids are largely cataions with net positive charge but they differ in the extent to which they are so ionized the cationic amino acids will therefore displace the bound Na+ ions from the resin particles at pH 3.0 the most basic amino acids [lysine, arginine, and the histidine] will displace Na+ first and will be bound to the resin most tightly and the most acids [glutamic acid, Serine] will be bound the least, the different amino acids will therefore move down the resin column at different rates the eluate fraction collected are **ysed quantitatively by means of the ninhydrin so that elution collection of fraction **ysis of each fraction and the recording  

          |--------150 cm column, pH 4.25, 0.2, N, Na, citrate----------| 

A        |       

B        |  

S        |                                        

O        |                                         Cysteine                      

R        |- 0.5                                         |                         Methionine      

B        |- 0.4                                         |                                 |               

A        |- 0.3                                         |                                 |                

N        |- 0.2                                         |                                 |              

C        |- 0.1                                         |                                 |                

E        |                                                |                                 |                        

          |-----|-----|------------|------------|--||------------|----------|-------|

                 40    80            280           320                  330          370

                                   Effluent, ml 

A = for example  

Numbers of Chromosomes in cell = 46.25

Methionine = C5H11NO2S = 151.75 M 

Methionine = C5H11NO2S = 151.75 M  

Human Chromosomes = 46.25

                                    a2 + b2 = c2 

                            Methionine = 151.75 M

                        Chromosomes = 46.25 M 

 = a2 + b2 = c2

 = a2 + b2 = c2 = Methionine + Chromosomes = c2   

 = a2 + b2 = c2 = a151.75 mm + b46.25 mm = c2    

 = a151.75 mm + b46.25 mm = c2  

 = a23028.0625 + b2139.0625 = c2  

 = a + b = 25167.125 = c2 

 = a + b = 25167.125 / 158.6 = 158.6

= c = 158.6 slope / 2 c2 = 79.3

      = 79.3 – 31.75 CH2OH = 47.55 

     = 47.55 - 1.55 = 46 

      = 46 = 2Na, Sodium

= c = 158.6 slope - 8.6 % Methionine = 150 cm column                 

Slope of c angle = 158.6 / 60 minute = 2.6433333333333333333333333333333

                       = 2.64333 seconds speed of slope and it has fast speed than the first triangle,  

Slope of c angle = 158.6 / 60 minute = 2.6433333333333333333333333333333

                       = 2.6433333333333333333333333333333 * 24 hours = 63.44

                       = 63.44 - 2.74 H2 of column = 60.7 

                       = 60.7 = Cross-proton of Methionine   

                       = 60.7 * 2.5 H2 = 151.75 mole Methionine    

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

By Time Algebra of Column of Methionine

                               Methionine = 151.75 M

                          Chromosomes = 46.25 M 

               Methionine = 151.75 * 24 hour = 3642

Human Chromosomes = 46.25 * 24 hour = 1110 

Total = 4752 * 5.29334 minute = 25153.95

        = 25153.96 / 158.6 = 158.6 slope

= c = 158.6 slope / 2 c2 = 79.3

      = 79.3 – 31.75 CH2OH = 47.55 

     = 47.55 - 1.55 = 46 

      = 46 = 2Na, Sodium

= c = 158.6 slope - 8.6 % Methionine = 150 cm column                 

The Methionine metabolism = 5.29334 minute