(Bio Mathematical Lab of Sikander Aqeel)
CHAPTER [3] PROTEINS
Proteins
(8/20/2016)
Charge and Chemical Properties of Amino acids and Proteins,
An understanding of protein requires knowledge of the ionizable side chain groups of the common amino acids, these ionizable groups common to proteins and amino acids, the acid form of the respective ionizable groups are on the left of the sum, while the respective properties base are on the right side, characteristic of the acid form is that nitrogen-containing groups are positively charged, whereas the acid forms that contain oxygen and sulfur atoms are natural,
Where acid group found Acid form = Base form,
C3H7NO2S = Cysteine R-SH = R-S + H+ = 8.0-9.0 pK
Thiol = Thiolate
Left side = C3H7NO2S = 122.75
Thiolate = R-S + H+ = 33.25
= a2 - b2 = c2
= a2 (Left side) + b2 (Thiolate) = c2
= a2 (122.75) + b2 (33.25) = c2
= a + b = (15067.5625) + b (1105.5625) = c2
= a + b = 16173.125 = c2
= a + b = 16173.125 / 127.17 = 127.17
= a + b = 127.17 / 1.5 Optimum pH of stomach pepsin = 84.78
= a + b = 84.78 / 2.649 Optimum pH of stomach peptide bond = 32
= a + b = 32 = S Thiolate at right side
= a + b = 33.25 = S + H+ = 33.25 = Thiolate at right side
= a + b = 33.25 / 4.156 hydrogen atoms = 8.0 pK
CHROMOSOMES WITH BIO-CHEMISTRY
Where acid group found Acid form = Base form,
C3H7NO2S = Cysteine R-SH = R-S + H+ = 8.0-9.0 pK
Thiol = Thiolate
Left side = C3H7NO2S = 122.75
Thiolate = R-S + H+ = 33.25
= a2 - b2 = c2
= a2 (Left side) + b2 (Thiolate) = c2
= a2 (122.75) + b2 (33.25) = c2
= a + b = (15067.5625) + b (1105.5625) = c2
= a + b = 16173.125 = c2
= a + b = 16173.125 / 127.17 = 127.17
= a + b = 127.17 / 1.5 Optimum pH of stomach pepsin = 84.78
= a + b = 84.78 / 2.649 Optimum pH of stomach peptide bond = 32
= a + b = 32 = S Thiolate at right side
= a + b = 44 = S + H+ = 33.25 = Thiolate at right side
= a + b = 33.25 / 4.156 hydrogen atoms = 8.0 pK
HUMAN CHROMOSOMES
Thiolate = R-S + H+ = 33.25
Human chromosomes = 46.25
= a2 + b2 = c2
= a2 (Thiolate) + b2 (chromosomes) = c2
= a2 (33.25) + b2 (46.25) = c2
= a + b = (1105.5625) + b (2139.0625) = c2
= a + b = 3244.625 = c2
= a + b = 3244.625 / 56.96 = 56.96
= a + b = 56.96 – 23.71 (H2O + H5) = 33.25
= a + b = 33.25 = S + H+ = base form at right side,
= a + b = 33.25 / 3.694 hydrogen atoms = 9.0 pK