BIO ALGEBRA OF LIVING ORGANISMS

(Bio Mathematical Lab of Sikander Aqeel) 

CHAPTER [3] PROTEINS  

Proteins  

Some Chemical Reactions of the Amino acids, 

The amino group in amino acid react with “ninhydrin” on heating to give a blue compound, as reaction is stoichiometric, the amount of amino aicds present in a solution can be quantified by the high absorption of wavelength 570 nm, Proline an amino acid, also react with ninhydrin, but gives a yellow color with a maximum absorbance at 440 nm, 

PROLINE AND NINHYDRIN  

1 = Proline = (C5H9NO2), 117.25 m/g

2 = Ninhydrin = (C9H6O4), 179.5 m/g

  = a2 + b2 = c2 

  = a2 (Proline) + b2 (Amino acid) = c2 

  = a2 (117.25) + b2 (179.5) = c2 

  = a + b = (13747.5625) + b (32220.25) = c2 

  = a + b = 45967.8125 = c2 

  = a + b = 45967.8125 / 45.25 (COOH) = 1015.86 

  = a + b = 1016 + 84 (Amino acid) = 1100

  = a + b = 1100 / 2.5 (H2 hydrin) = 440 

  = a + b = 440 (nm) yellow color 

They give a yellow color with a maximum absorbance at 440 nm 

HUMAN CHROMOSOMES

PROLINE AND NINHYDRIN WITH CHROMOSOMES   

1 = Proline = (C5H9NO2), 117.25 m/g

2 = Ninhydrin = (C9H6O4), 179.5 m/g 

3 = human Chromosomes = 46.25

  = a2 + b2 = c2 

  = a2 (Proline) + b2 (Amino acid) = c2 

  = a2 (117.25) + b2 (179.5) = c2 

  = a + b = (13747.5625) + b (32220.25) = c2 

  = a + b = 45967.8125 = c2 

  = a + b = 45967.8125 / 46.25 (Chromosomes) = 993.89

  = a + b = 994 + 106 (Serine) = 1100

  = a + b = 1100 / 2.5 (H2 hydrin) = 440 

  = a + b = 440 (nm) yellow color 

They give a yellow color with a maximum absorbance at 440 nm