BIO ALGEBRA OF LIVING ORGANISMS

(Bio Mathematical Lab of Sikander Aqeel) 

CHAPTER [3] PROTEINS  

Proteins  

TECHNIQUES UTILIZED TO DETERMINE THE AMINO ACID SEQUENCE OF A PROTEIN  

Knowledge of the primary structure (amino acid sequence) of a protein is required for an understanding of the relationship of a protein’s structure to its function on a molecular level, 

In the determination of primary structure, first the number of polypeptide chain in the protein must be ascertained, to begin with the protein is denatured, and the treated with a reagent, such as Insulin, that forms a covalent bond with the NH2-termina a-amino groups of each polypeptide chain within the protein the tagged protein is then hydrolyzed to its constituent amino acids, typically condition for complete protein hydrolysis are 6 N HCl at 110 centigrade for 18 to 36 hours, in a sealed tube under vacuum, the vacuum prevents degradation of oxidation sensitive,      

PROTEIN HYDROLYSIS (10/16/2016) 

GHRELIN 

1 = Peptide Hormone = 2817.94 = 28 amino acids of Ghrelin

2 = Centigrade = 110 

  = a2 / b2 = c2 

  = a2 (Peptide Hormone) / b2 (centigrade) = c2 

  = a2 (2817.94)             / b2 (110) = c2 

  = a / b = (7940785.84) / b (12100) = c2 

  = a / b = 656.26 = c2 

  = a / b = 656.26 - 107.26 % cell membarne = 549

  = a / b = 549 = (C6H12O6)3 glucose level 

PROTEIN HYDROLYSIS  

GHRELIN 

1 = Peptide Hormone = 2817.94 = 28 amino acids of Ghrelin

2 = Centigrade = 110 

  = a2 + b2 = c2 

  = a2 (Peptide Hormone) + b2 (centigrade) = c2 

  = a2 (2817.94)         + b2 (110) = c2 

  = a + b = (7940785.84) + b (12100) = c2 

  = a + b = 7952885.84 = c2 

  = a + b = 7952885.84 / 2820.08 = 2820.09

  = a + b = 2820.09 / 2.5 (H2= A//T DNA) = 1128.036

  = a + b = 1128.036 / 3.75 (H3= C///G DNA) = 300.8096

  = a + b = 300.81 = Thiamine or Vitamin-B1 to increase hunger     

HUMAN CHROMOSOMES 

GHRELIN 

1 = Peptide Hormone = 2817.94 = 28 amino acids of Ghrelin

2 = Human Chromosomes = 46.25 

  = a2 + b2 = c2 

  = a2 (Peptide Hormone) + b2 (Chromosomes) = c2 

  = a2 (2817.94)         + b2 (46.25) = c2 

  = a + b = (7940785.84) + (2139.0625) = c2 

  = a + b = 7942924.9025 = c2 

  = a + b = 7942924.9025 / 2818.31 = 2818.32

  = a + b = 2818.32 / 2.5 (H2= A//T DNA) = 1127.328

  = a / b = 1127.328 / 3.75 (H3= C///G DNA) = 300.62

  = a / b = 300.62 + 0.19 = 300.81

  = a / b = 300.81 = Thiamine or Vitamin-B1 to increase hunger 

1 = Peptide Hormone = 2817.94 / 100 % cell membarne = 28.1794 amino acids of Ghrelin

Stimulates release of Growth Hormone, increases feeling of hunger.